Article
Cloning and expression of pigeon liver cytosolic NADP(+)-dependent malic enzyme cDNA and some of its abortive mutants.
Archives of biochemistry and biophysics - 1 Apr 1994
Chou W Y, Huang S M, Liu Y H, Chang G G
Abstract excerpt
A full-length 1927-base-pair cDNA of pigeon liver malic enzyme was obtained by utilizing the screening of the cDNA library and polymerase chain reaction techniques. The cDNA contained one open reading frame coding for 557 amino acid residues, flanked by 86 and 167 nucleotides at the 5' and 3' ter...
Topics
- Adenosine Diphosphate
- Amino Acid Sequence
- Animals
- Base Sequence
- Chromatography, Affinity
- Cloning, Molecular
- Columbidae
- Cytosol
- DNA Probes
- DNA, Complementary
- Escherichia coli
- Gene Library
- Liver
- Malate Dehydrogenase
- Molecular Sequence Data
- Mutation
- NADP
- Protein Conformation