Article
Genetic variability of the PRNP gene in goat breeds from Northern and Southern Italy.
Journal of applied microbiology - 1 Jun 2008
Acutis P L, Colussi S, Santagada G, Laurenza C, Maniaci M G, Riina M V, Peletto S, Goldmann W, Bossers A, Caramelli M, Cristoferi I, Maione S, Sacchi P, Rasero R
Abstract excerpt
AIMS: To determine the variability of the prion protein gene (PRNP) in goats from Northern and Southern Italy. METHODS AND RESULTS: Genomic DNA isolated from goat blood was polymerase chain reaction (PCR)-amplified for the coding region of the PRNP gene and then sequenced. In total, 13 polymorphic sites were identified: G37V, T110P, G127S, M137I, I142M, I142T, H143R, R154H, P168Q, T194P, R211Q, Q222K and S240P...
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