Article
Development of JFH1-based cell culture systems for hepatitis C virus genotype 4a and evidence for cross-genotype neutralization.
Proceedings of the National Academy of Sciences of the United States of America - 22 Jan 2008
Scheel Troels K H, Gottwein Judith M, Jensen Tanja B, Prentoe Jannick C, Hoegh Anne M, Alter Harvey J, Eugen-Olsen Jesper, Bukh Jens
Abstract excerpt
Efficient in vitro systems to study the life cycle of hepatitis C virus (HCV) were recently developed for JFH1 (genotype 2a), which has unique replication capacity in Huh7 cells. We developed 4a/JFH1 intergenotypic recombinants containing the structural genes (Core, E1, and E2), p7, and all or part of NS2 of the 4a prototype strain ED43 that, after transfection of Huh7.5 cells with RNA transcripts, produced...
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