Article
Regulation of rat dopamine beta-hydroxylase gene transcription by early growth response gene 1 (Egr1).
Brain research - 8 Feb 2008
Cheng Shu-Yuan, Serova Lidia I, Glazkova Dina, Sabban Esther L
Abstract excerpt
Egr1, a transcription factor rapidly induced by various stimuli including stress, can elevate transcription of genes for the catecholamine biosynthetic enzymes TH and PNMT. To examine if Egr1 also regulates dopamine beta-hydroxylase (DBH) gene expression, PC12 cells were transfected with expression vector for full length or truncated inactive Egr1 and various DBH promoter-driven luciferase constructs. While Egr1...
Topics
- Analysis of Variance
- Animals
- Chromatin Immunoprecipitation
- Cyclic AMP
- Dopamine beta-Hydroxylase
- Early Growth Response Protein 1
- Electrophoresis, Polyacrylamide Gel
- Electrophoretic Mobility Shift Assay
- Gene Expression Regulation
- Luciferases
- Mutation
- PC12 Cells
- Promoter Regions, Genetic