Article
Secretion and in vivo folding of the Fab fragment of the antibody McPC603 in Escherichia coli: influence of disulphides and cis-prolines.
Protein engineering - 1 Dec 1991
Skerra A, Plückthun A
Abstract excerpt
Using the well-characterized antibody McPC603 as a model, we had found that the Fv fragment can be isolated from Escherichia coli as a functional protein in good yields, whereas the amount of the correctly folded Fab fragment of the same antibody produced under identical conditions is significantly lower. In this paper, we analyse the reasons for this difference. We found that a variety of signal sequences...
Topics
- Amino Acid Sequence
- Animals
- Antibodies, Monoclonal
- Disulfides
- Escherichia coli
- Gene Expression Regulation, Bacterial
- Immunoglobulin A
- Immunoglobulin Fab Fragments
- Immunoglobulin Switch Region
- Isomerism
- Mice