Article
Agonist- and competitive antagonist-induced movement of loop 5 on the alpha subunit of the neuronal alpha4beta4 nicotinic acetylcholine receptor.
Journal of neurochemistry - 1 Apr 2008
Mourot Alexandre, Bamberg Ernst, Rettinger Jürgen
Abstract excerpt
Neuronal nicotinic acetylcholine receptors (nAChRs) are ligand-gated ion channels that rapidly convert a chemical signal into an electrical signal. Although the structure of the nAChR is quite well described, the coupling between agonist binding and channel gating is still under debate. In this study, we probed local conformational transitions on the neuronal alpha4beta4 nAChR by specifically tethering a...
Topics
- Acetylcholine
- Animals
- Cysteine
- Dihydro-beta-Erythroidine
- Membrane Potentials
- Microinjections
- Molecular Biology
- Mutation
- Nicotinic Agonists
- Nicotinic Antagonists
- Oocysts
- Patch-Clamp Techniques
- Protein Subunits
- Receptors, Nicotinic
- Rhodamines
- Xenopus