Article
Variants of human seminal acrosin inhibitor (HUSI-II) which inhibit human leukocyte elastase.
Biomedica biochimica acta - 1 Jan 1991
Collins J, Taube W, Fink E, Möritz A, Fritz H
Abstract excerpt
A cDNA coding for human seminal inhibitor II was fused to the ompA-gene leader peptide for the expression in E. coli. The secreted protein is biologically active and correctly processed. Inhibitors of human leukocyte elastase were constructed by site-directed mutagenesis.
Topics
- Amino Acid Sequence
- Cloning, Molecular
- Escherichia coli
- Genetic Variation
- Humans
- In Vitro Techniques
- Leukocyte Elastase
- Male
- Molecular Sequence Data
- Mutagenesis, Site-Directed
- Pancreatic Elastase
- Proteinase Inhibitory Proteins, Secretory
- Proteins
- Recombinant Fusion Proteins
- Semen
- Serine Proteinase Inhibitors
