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Article

Anti-parallel membrane topology of a homo-dimeric multidrug transporter, EmrE.

Journal of biochemistry - 1 Nov 2007

Nara Toshifumi, Kouyama Tomoko, Kurata Yuko, Kikukawa Takashi, Miyauchi Seiji, Kamo Naoki

Abstract excerpt

EmrE in Escherichia coli belongs to the small multidrug resistance (SMR) transporter family. It functions as a homo-dimer, but the orientation of the two monomers in the membrane (membrane topology) is under debate. We expressed various single-cysteine EmrE mutants in E. coli cells lacking a major efflux transporter. Efflux from cells expressing the P55C or T56C mutant was blocked by the external application of...

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Identifiers

PMID: 18006522
DOI: 10.1093/jb/mvm169

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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