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Article

Fine-tuning the topology of a polytopic membrane protein: role of positively and negatively charged amino acids.

Cell - 21 Sept 1990

Nilsson I, von Heijne G

Abstract excerpt

The effects of positively and negatively charged residues on the membrane topology of a model E. coli protein with two transmembrane segments have been studied. We show that addition or removal of as little as a single positively charged lysine residue in one of two critical regions can be sufficient to reverse the transmembrane topology of the molecule from Nout-Cout to Nin-Cin. Negatively charged residues are...

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Identifiers

PMID: 2119256
DOI: 10.1016/0092-8674(90)90390-z

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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