Article
Fine-tuning the topology of a polytopic membrane protein: role of positively and negatively charged amino acids.
Cell - 21 Sept 1990
Nilsson I, von Heijne G
Abstract excerpt
The effects of positively and negatively charged residues on the membrane topology of a model E. coli protein with two transmembrane segments have been studied. We show that addition or removal of as little as a single positively charged lysine residue in one of two critical regions can be suffic...
Topics
- Amino Acid Sequence
- Amino Acids, Diamino
- Amino Acids, Dicarboxylic
- Cell Membrane
- Endopeptidases
- Escherichia coli
- Lysine
- Membrane Proteins
- Molecular Sequence Data
- Mutation
- Plasmids
- Protein Conformation
- Serine Endopeptidases