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Article

Humanization of a mouse monoclonal antibody by CDR–grafting: the importance of framework residues on loop conformation

1 Jan 1991

Abstract excerpt

A mouse monoclonal antibody (mAb 425) with therapeutic potential was 'humanized' in two ways. Firstly the mouse variable regions from mAb 425 were spliced onto human constant regions to create a chimeric 425 antibody. Secondly, the mouse complementarity-determining regions (CDRs) from mAb 425 were grafted into human variable regions, which were then joined to human constant regions, to create a reshaped human 425...

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PMID: 1798701
DOI: 10.1093/protein/4.7.773

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