Article
Block copolymer-oligonucleotide conjugates for genotyping on microarrays.
Analytical biochemistry - 15 Feb 2008
de Lambert Bertrand, Chaix Carole, Charreyre Marie-Thérèse, Martin Thibault, Aigoui Arnaud, Perrin-Rubens Agnès, Pichot Christian, Mandrand Bernard
Abstract excerpt
Polymer-oligonucleotide conjugates were synthesized from the amphiphilic block copolymer poly(tert-butylacrylamide-b-(N-acryloylmorpholine-co-N-acryloxysuccinimide)) using an original solid-phase DNA synthesis strategy. This method provided conjugates highly functionalized with oligonucleotides throughout the polymer chain. After purification, block copolymer-oligonucleotide conjugates were spotted on a...
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