Article
Preparation of enzymes required for enzymatic quantification of 5-keto-D-gluconate and 2-keto-D-gluconate.
Bioscience, biotechnology, and biochemistry - 1 Oct 2007
Saichana Ittipon, Ano Yoshitaka, Adachi Osao, Matsushita Kazunobu, Toyama Hirohide
Abstract excerpt
For easy measurement of 5-keto D-gluconate (5KGA) and 2-keto D-gluconate (2KGA), two enzymes, 5KGA reductase (5KGR) and 2KGA reductase (2KGR) are useful. The gene for 5KGR has been reported, and a corresponding gene was found in the genome of Gluconobacter oxydans 621H and was identified as GOX2187. On the other hand, the gene for 2KGR was identified in this study as GOX0417 from the N-terminal amino acid...
Topics
- Amino Acid Sequence
- Carbohydrate Dehydrogenases
- Cloning, Molecular
- Enzyme Stability
- Escherichia coli
- Flavin-Adenine Dinucleotide
- Genes, Bacterial
- Gluconates
- Gluconobacter
- Models, Biological
- Molecular Sequence Data
- Mutation
- Plasmids
- Sequence Homology, Amino Acid