Article
Membrane-bound, 2-keto-D-gluconate-yielding D-gluconate dehydrogenase from "Gluconobacter dioxyacetonicus" IFO 3271: molecular properties and gene disruption.
Applied and environmental microbiology - 1 Oct 2007
Toyama Hirohide, Furuya Naoko, Saichana Ittipon, Ano Yoshitaka, Adachi Osao, Matsushita Kazunobu
Abstract excerpt
Most Gluconobacter species produce and accumulate 2-keto-d-gluconate (2KGA) and 5KGA simultaneously from d-glucose via GA in culture medium. 2KGA is produced by membrane-bound flavin adenine dinucleotide-containing GA 2-dehydrogenase (FAD-GADH). FAD-GADH was purified from "Gluconobacter dioxyacetonicus" IFO 3271, and N-terminal sequences of the three subunits were analyzed. PCR primers were designed from the...
Topics
- Amino Acid Sequence
- Carbohydrate Dehydrogenases
- Cell Membrane
- Flavin-Adenine Dinucleotide
- Gluconates
- Gluconobacter
- Molecular Sequence Data
- Mutation
- Polymerase Chain Reaction
- Sequence Analysis, DNA