Article
Characterization of enzymes in the oxidation of 1,2-propanediol to D: -(-)-lactic acid by Gluconobacter oxydans DSM 2003.
Molecular biotechnology - 1 Sept 2010
Wei Liujing, Yang Xuepeng, Gao Keliang, Lin Jinping, Yang Shengli, Hua Qiang, Wei Dongzhi
Abstract excerpt
Although Gluconobacter oxydans can convert 1,2-propanediol to D: -(-)-lactic acid, the enzyme(s) responsible for the conversion has remain unknown. In this study, the membrane-bound alcohol dehydrogenase (ADH) of Gluconobacter oxydans DSM 2003 was purified and confirmed to be essential for the process of D: -(-)-lactic acid production by gene knockout and complementation studies. A 25 percent decrease in D:...
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