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Metabolic engineering of Gluconobacter oxydans for improved growth rate and growth yield on glucose by elimination of gluconate formation.

Applied and environmental microbiology - 1 Jul 2010

Krajewski Vera, Simic Petra, Mouncey Nigel J, Bringer Stephanie, Sahm Hermann, Bott Michael

Abstract excerpt

Gluconobacter oxydans N44-1, an obligatory aerobic acetic acid bacterium, oxidizes glucose primarily in the periplasm to the end products 2-ketogluconate and 2,5-diketogluconate, with intermediate formation of gluconate. Only a minor part of the glucose (less than 10%) is metabolized in the cytoplasm after conversion to gluconate or after phosphorylation to glucose-6-phosphate via the only functional catabolic...

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PMID: 20453146
DOI: 10.1128/AEM.03022-09
PMCID: PMC2897456

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