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Article

Proximity-Triggered Covalent Stabilization of Low-Affinity Protein Complexes In Vitro and In Vivo.

Angewandte Chemie (International ed. in English) - 4 Dec 2017

Cigler Marko, Müller Thorsten G, Horn-Ghetko Daniel, von Wrisberg Marie-Kristin, Fottner Maximilian, Goody Roger S, Itzen Aymelt, Müller Matthias P, Lang Kathrin

Abstract excerpt

The characterization of low-affinity protein complexes is challenging due to their dynamic nature. Here, we present a method to stabilize transient protein complexes in vivo by generating a covalent and conformationally flexible bridge between the interaction partners. A highly active pyrrolysyl tRNA synthetase mutant directs the incorporation of unnatural amino acids bearing bromoalkyl moieties (BrCnK) into...

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PMID: 28960788
DOI: 10.1002/anie.201706927

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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