Article
A new polymerase chain reaction/restriction fragment length polymorphism protocol for Plasmodium vivax circumsporozoite protein genotype (VK210, VK247, and P. vivax-like) determination.
Diagnostic microbiology and infectious disease - 1 Dec 2007
Alves Renata Tomé, Póvoa Marinete Marins, Goldman Ira F, Cavasini Carlos Eugênio, Rossit Andréa Regina Baptista, Machado Ricardo Luiz Dantas
Abstract excerpt
For the molecular diagnosis of Plasmodium vivax variants (VK210, VK247, and P. vivax-like) using DNA amplification procedures in the laboratory, the choice of rapid and inexpensive identification products of the 3 different genotypes is an important prerequisite. We report here the standardization of a new polymerase chain reaction/restriction fragment length polymorphism technique to identify the 3 described P....
Topics
- Animals
- Genotype
- Humans
- Malaria, Vivax
- Parasitology
- Plasmodium vivax
- Polymerase Chain Reaction
- Polymorphism, Restriction Fragment Length
- Prevalence
- Protozoan Proteins
- Sensitivity and Specificity