Article
[Studies on identification of circumsporozoite protein genotyping of Plasmodium vivax].
Zhongguo ji sheng chong xue yu ji sheng chong bing za zhi = Chinese journal of parasitology & parasitic diseases - 1 Jan 2000
Huang T Y, Wang X L, Li X M, Huang Y M, Zeng F X, Che Y, Zhang S M, Fu W Z, Zhang Z X, Zhang G S, Cai X Z, Wang S Q, Wang G Z
Abstract excerpt
OBJECTIVE: To develop a new method of genotyping circumsporozoite protein (CSP) gene for identification of field isolates of Plasmodium vivax. METHODS: Improved Chelex-100 ion-exchange method was used to extract DNA from blood filter paper samples, nested PCR and allele-specific PCR techniques, agarose gel electrophoresis analysis and dot/southern blotting-probe hybridization were employed for amplification,...
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