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Article

A system for the directed evolution of the insecticidal protein from Bacillus thuringiensis.

Molecular biotechnology - 1 Jun 2007

Ishikawa Hiroshi, Hoshino Yasushi, Motoki Yutaka, Kawahara Takuma, Kitajima Mika, Kitami Madoka, Watanabe Ayako, Bravo Alejandra, Soberon Mario, Honda Atsuko, Yaoi Katsuro, Sato Ryoichi

Abstract excerpt

Theoretically, the activity of AB-type toxin molecules such as the insecticidal toxin (Cry toxin) from B. thuringiensis, which have one active site and two binding site, is improved in parallel with the binding affinity to its receptor. In this experiment, we tried to devise a method for the directed evolution of Cry toxins to increase the binding affinity to the insect receptor. Using a commercial T7...

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PMID: 17914188
DOI: 10.1007/s12033-007-0001-9

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