Article
Cloning of a new cryIA(a) gene from Bacillus thuringiensis strain FU-2-7 and analysis of chimaeric CryIA(a) proteins for toxicity.
Bioscience, biotechnology, and biochemistry - 1 May 1994
Udayasuriyan V, Nakamura A, Mori H, Masaki H, Uozumi T
Abstract excerpt
We cloned the cryIA(a) gene from Bacillus thuringiensis strain FU-2-7, one of the toxin genes encoding lepidopteran-specific protoxins. Sequence analysis of the gene showed two amino acid differences (Pro77 to Leu and Phe965 to Ser) from the CryIA(a) of B. thuringiensis strain HD-1. We constructe...
Topics
- Amino Acid Sequence
- Animals
- Bacillus thuringiensis
- Base Sequence
- Biotechnology
- Bombyx
- Cloning, Molecular
- DNA
- Escherichia coli
- Genes, Bacterial
- Genetic Variation
- Insecta
- Molecular Sequence Data
- Recombinant Fusion Proteins
- Restriction Mapping
- Species Specificity