Article
Expression of Cry1Ac toxin-binding region in Plutella xyllostella cadherin-like receptor and studying their interaction mode by molecular docking and site-directed mutagenesis.
International journal of biological macromolecules - 1 May 2018
Hu Xiaodan, Zhang Xiao, Zhong Jianfeng, Liu Yuan, Zhang Cunzheng, Xie Yajing, Lin Manman, Xu Chongxin, Lu Lina, Zhu Qing, Liu Xianjin
Abstract excerpt
Cadherin-like protein has been identified as the primary Bacillus thuringiensis (Bt) Cry toxin receptor in Lepidoptera pests and plays a key role in Cry toxin insecticidal. In this study, we successfully expressed the putative Cry1Ac toxin-binding region (CR7-CR11) of Plutella xylostella cadherin-like in Escherichia coli BL21 (DE3). The expressed CR7-CR11 fragment showed binding ability to Cry1Ac toxin under...
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