Article
Construction and characterization of Bordetella pertussis mutants lacking the vir-regulated P.69 outer membrane protein.
Molecular microbiology - 1 Jun 1991
Roberts M, Fairweather N F, Leininger E, Pickard D, Hewlett E L, Robinson A, Hayward C, Dougan G, Charles I G
Abstract excerpt
The Bordetella pertussis P.69 protein is an immunogen with vaccine potential. The role of this protein in pathogenesis is unclear; it has been associated with the toxic adenylate cyclase and adhesion to eukaryotic cells. For further analysis of the role of P.69 in the biology of B. pertussis, we have constructed strains which specifically lack P.69. The cloned P.69 (prn) gene of B. pertussis was insertionally...
Topics
- Adenylyl Cyclases
- Adhesins, Bacterial
- Agglutination Tests
- Animals
- Bacterial Adhesion
- Bacterial Outer Membrane Proteins
- Blotting, Southern
- Blotting, Western
- Bordetella pertussis
- Cell Line