Article
A new assay for invasion of HeLa 229 cells by Bordetella pertussis: effects of inhibitors, phenotypic modulation, and genetic alterations.
Infection and immunity - 1 Aug 1990
Lee C K, Roberts A L, Finn T M, Knapp S, Mekalanos J J
Abstract excerpt
Invasion and intracellular survival of Bordetella pertussis in HeLa 229 cells was studied by a new assay that utilizes polymyxin B instead of gentamicin to rapidly kill extracellular organisms. Invasion measured by this assay was time and temperature dependent and was inhibited by the microfilame...
Topics
- Bacterial Proteins
- Bordetella Infections
- Bordetella pertussis
- Cytochalasin D
- Electrophoresis, Polyacrylamide Gel
- Gene Expression
- HeLa Cells
- Hemagglutinins
- Humans
- Mutation
- Phenotype
- Plasmids
- Polymyxin B
- Temperature
- Virulence
- Virulence Factors