Article
Contributions of intracellular loops 2 and 3 of the lutropin receptor in Gs coupling.
Molecular endocrinology (Baltimore, Md.) - 1 Jan 2008
Angelova Krassimira, Fanelli Francesca, Puett David
Abstract excerpt
A number of amino acids essential for Gs coupling, i.e. hot spots, were identified after in vitro Ala-scanning mutagenesis of the cytosolic extensions of helices 3, 5, and 6 and of intracellular loops 2 and 3 (IL2 and IL3) of the human LH receptor (LHR). Consistent with the results of in vitro experiments involving ligand binding and ligand-mediated signaling in transiently transfected human embryonic kidney 293...
Topics
- Amino Acid Sequence
- Binding Sites
- Cell Line
- Computer Simulation
- Cyclic AMP
- GTP-Binding Proteins
- Humans
- Models, Molecular
- Molecular Sequence Data
- Mutagenesis, Site-Directed
- Mutation
- Protein Binding
- Protein Structure, Secondary
- Protein Structure, Tertiary