Article
Insight into mutation-induced activation of the luteinizing hormone receptor: molecular simulations predict the functional behavior of engineered mutants at M398.
Molecular endocrinology (Baltimore, Md.) - 1 Jun 2004
Fanelli Francesca, Verhoef-Post Miriam, Timmerman Marianna, Zeilemaker Annelieke, Martens John W M, Themmen Axel P N
Abstract excerpt
In this study, molecular simulations have been combined with site-directed mutagenesis experiments to explore M398(2.43), a LH (lutropin) receptor (LHR) site in helix 2 susceptible to spontaneous activating mutations, and to develop a computational tool for predicting the functionality (i.e. active or nonactive) of LHR mutants.Site-directed mutagenesis experiments engineered 15 different substitutions for...
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