Article
Production of antisense RNA leads to effective and specific inhibition of gene expression in C. elegans muscle.
Development (Cambridge, England) - 1 Oct 1991
Fire A, Albertson D, Harrison S W, Moerman D G
Abstract excerpt
We have used an antisense strategy to effectively disrupt the expression of two genes encoding myofilament proteins present in C. elegans body wall muscles. DNA segments from the unc-22 and unc-54 genes have been placed in reverse orientation in vectors designed to produce RNA in body wall muscles. When the resulting plasmids are injected into oocytes, progeny with defects in muscle function are produced. These...
Topics
- Animals
- Caenorhabditis
- Gene Expression
- Gene Expression Regulation
- Microfilament Proteins
- Muscles
- Phenotype
- RNA, Antisense