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Article

CRISPR-mediated Transfection of Brugia malayi

31 Aug 2020

Abstract excerpt

The application of reverse genetics in the human filarial parasites has lagged due to the difficult biology of these organisms. Recently, we developed a co-culture system that permitted the infective larval stage of Brugia malayi to be transfected and efficiently develop to fecund adults. This was exploited to develop a piggyBac transposon-based toolkit that can be used to produce parasites with transgene...

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PMID: 32866158
DOI: 10.1371/journal.pntd.0008627

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