Article
Use of a Gluconobacter frateurii mutant to prevent dihydroxyacetone accumulation during glyceric acid production from glycerol.
Bioscience, biotechnology, and biochemistry - 1 Jan 2010
Habe Hiroshi, Shimada Yuko, Fukuoka Tokuma, Kitamoto Dai, Itagaki Masayuki, Watanabe Kunihiro, Yanagishita Hiroshi, Yakushi Toshiharu, Matsushita Kazunobu, Sakaki Keiji
Abstract excerpt
To prevent dihydroxyacetone (DHA) by-production during glyceric acid (GA) production from glycerol using Gluconobacter frateurii, we used a G. frateurii THD32 mutant, ΔsldA, in which the glycerol dehydrogenase subunit-encoding gene (sldA) was disrupted, but ΔsldA grew much more slowly than the wild type, growth starting after a lag of 3 d under the same culture conditions. The addition of 1% w/v D-sorbitol to the...
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