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Article

Refolding of human beta-1-2 GlcNAc transferase (GnT1) and the role of its unpaired Cys 121.

Biochemical and biophysical research communications - 19 Oct 2007

Saribas A Sami, Johnson Karl, Liu Li, Bezila Dan, Hakes David

Abstract excerpt

Human beta1-2N-acetylglucosaminyltransferase (hGnT1) lacking the first 103 amino acids was expressed as a maltose binding protein (MBP) fusion protein in inclusion bodies (IBs) in Escherichia coli and refolded using an oxido-shuffling method. GnT1 mutants were prepared by replacing a predicted unpaired cysteine (C121) with alanine (C121A), serine (C121S), threonine (C121T) or aspartic acid (C121D). A double...

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Identifiers

PMID: 17716624
DOI: 10.1016/j.bbrc.2007.07.199

Genes

MBP

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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