Article
New markers for murine memory B cells that define mutated and unmutated subsets.
The Journal of experimental medicine - 3 Sept 2007
Anderson Shannon M, Tomayko Mary M, Ahuja Anupama, Haberman Ann M, Shlomchik Mark J
Abstract excerpt
The study of murine memory B cells has been limited by small cell numbers and the lack of a definitive marker. We have addressed some of these difficulties with hapten-specific transgenic (Tg) mouse models that yield relatively large numbers of antigen-specific memory B cells upon immunization. Using these models, along with a 5-bromo-2'-deoxyuridine (BrdU) pulse-label strategy, we compared memory cells to their...
Topics
- Animals
- B-Lymphocytes
- B7-1 Antigen
- Base Sequence
- Biomarkers
- Bromodeoxyuridine
- Cell Survival
- DNA
- Immunity, Cellular
- Immunization
- Immunologic Memory
- Lymphocyte Subsets
- Mice
- Mice, Transgenic
- Mutation