The use of proline as a nitrogen source causes hypersensitivity to, and allows more economical use of 5FOA in Saccharomyces cerevisiae.

doi:10.1002/yea.320070608

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Article

The use of proline as a nitrogen source causes hypersensitivity to, and allows more economical use of 5FOA in Saccharomyces cerevisiae.

Yeast (Chichester, England) - 1 Jan 2000

McCusker J H, Davis R W

Abstract excerpt

The use of proline as a nitrogen source causes hypersensitivity to 5-fluoro-orotic acid (5FOA) and allows up to 40-fold less of this drug to be used to select for the loss of URA3 function in Saccharomyces cerevisiae. 5FOA hypersensitivity is presumably due to the absence of nitrogen catabolite r...

Topics

Culture Media
Mutation
Orotic Acid
Proline
Saccharomyces cerevisiae
Uracil

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