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Article

Rate-limiting steps and role of active site Lys443 in the mechanism of carbapenam synthetase.

Biochemistry - 14 Aug 2007

Arnett Samantha O, Gerratana Barbara, Townsend Craig A

Abstract excerpt

Carbapenam synthetase (hereafter named CPS) catalyzes the formation of the beta-lactam ring in the biosynthetic pathway to (5R)-carbapen-2-em-3-carboxylate, the simplest of the carbapenem antibiotics. Kinetic studies showed remarkable tolerance to substrate stereochemistry in the turnover rate but did not distinguish between chemistry and a nonchemical step such as product release or conformational change as...

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Identifiers

PMID: 17658887
DOI: 10.1021/bi0618464
PMCID: PMC3198785

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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