Article
Pathway engineering results the altered polyhydroxyalkanoates composition in recombinant Escherichia coli.
New biotechnology - 31 Jan 2011
Li Qiang, Chen Quan, Li Ming-Ji, Wang Feng-Shan, Qi Qing-Sheng
Abstract excerpt
To efficiently produce short-chain-length-medium-chain-length polyhydroxyalkanoates copolymer from substrate mixture containing sugars and/or fatty acids, fadA gene mutant was constructed in Escherichia coli DH5α phosphotransferase system (PTS) disrupted strain. Plasmids pCJY02, pBHR68 and pBHR71 were separately introduced into E. coli DH5α (ΔptsG, ΔFadA) by transformation, then the recombinants were cultivated...
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