Article
A new beneficial mutation in pseudomonas sp. 61-3 polyhydroxyalkanoate (PHA) synthase for enhanced cellular content of 3-hydroxybutyrate-based PHA explored using its enzyme homolog as a mutation template.
Bioscience, biotechnology, and biochemistry - 1 Jan 2010
Shozui Fumi, Sun Jian, Song Yuyang, Yamada Miwa, Sakai Kohei, Matsumoto Ken'ichiro, Takase Kazuma, Taguchi Seiichi
Abstract excerpt
A newly isolated mutation (Gln508Leu) and a combination of it with previously discovered beneficial mutations in polyhydroxyalkanoate synthase 1 from Pseudomonas sp. 61-3 were found to enhance the production of poly(3-hydroxybutyrate) [P(3HB)] and poly(3HB-co-3-hydroxyalkanoate)s in recombinant E...
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