Housekeeping gene validation for RT-qPCR studies on synovial fibroblasts derived from healthy and osteoarthritic patients with focus on mechanical loading

Abstract excerpt

Selection of appropriate housekeeping genes is essential for the validity of data normalization in reverse transcription quantitative PCR (RT-qPCR). Synovial fibroblasts (SF) play a mediating role in the development and progression of osteoarthritis (OA) pathogenesis, but there is no information on reliable housekeeping genes available. Therefore the goal of this study was to identify a set of reliable...

Topics

Share this publication in a Topic to start or enrich a Post.

• Share to Molecular Biology Techniques and Applications
• Share to Osteoarthritis Treatment and Mechanisms
• Share to Viral Infections and Immunology Research