Determination of novel reference genes for improving gene expression data normalization in selected canine reproductive tissues – a multistudy analysis

Abstract excerpt

BACKGROUND: Real time RT-PCR (qPCR) is a useful and powerful tool for quantitative measurement of gene expression. The proper choice of internal standards such as reference genes is crucial for correct data evaluation. In female dogs, as in other species, the reproductive tract is continuously undergoing hormonal and cycle stage-dependent morphological changes, which are associated with altered gene expression....

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