Article
Evaluation of PorB variable region typing of Neisseria gonorrhoeae using PCR-ELISA in samples collected from men who have sex with men.
Journal of clinical laboratory analysis - 1 Jan 2007
Ling A E, Bash M C, Lynn F, Lister N A, Zhu P, Garland S M, Fairley C K, Tabrizi S N
Abstract excerpt
A polymerase chain reaction (PCR)-based enzyme-linked immunosorbent assay (ELISA) methodology was developed to characterize Neisseria gonorrhoeae porB gene variable regions (VR); the methodology was evaluated in comparison to porB VR typing by checkerboard hybridization. Clinical noncultured samples from 35 men who have sex with men (MSM), positive by nucleic amplification assays for N. gonorrhoeae, were typed...
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