Article
Determination of the secondary structure in solution of the Escherichia coli DnaA DNA-binding domain.
Biochemical and biophysical research communications - 22 Nov 2002
Obita Takayuki, Iwura Takafumi, Su'etsugu Masayuki, Yoshida Yoichiro, Tanaka Yoshitsugu, Katayama Tsutomu, Ueda Tadashi, Imoto Taiji
Abstract excerpt
DnaA protein binds specifically to a group of binding sites collectively called as DnaA boxes within the bacterial replication origin to induce local unwinding of duplex DNA. The DNA-binding domain of DnaA, domain IV, comprises the C-terminal 94 amino acid residues of the protein. We overproduced and purified a protein containing only this domain plus a methionine residue. This protein was stable as a monomer and...
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