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Y chromosome detection by Real Time PCR and pyrophosphorolysis‐activated polymerisation using free fetal DNA isolated from maternal plasma

29 Jun 2007

Abstract excerpt

OBJECTIVES: To validate the use of Real Time PCR, a widely used technique that can detect very low levels of Y chromosomal sequence, and to assess the use of a highly sensitive PCR technique, pyrophosphorolysis-activated polymerisation (PAP), for fetal sex determination using free fetal DNA (ffDNA). METHODS: The fetal sex was determined by Real Time PCR in 58 pregnancies using ffDNA isolated from maternal plasma....

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PMID: 17600849
DOI: 10.1002/pd.1804

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