Article
Production and secretion stress caused by overexpression of heterologous alpha-amylase leads to inhibition of sporulation and a prolonged motile phase in Bacillus subtilis.
Applied and environmental microbiology - 1 Aug 2007
Lulko Andrzej T, Veening Jan-Willem, Buist Girbe, Smits Wiep Klaas, Blom Evert Jan, Beekman Aaron C, Bron Sierd, Kuipers Oscar P
Abstract excerpt
Transcriptome analysis was used to investigate the global stress response of the gram-positive bacterium Bacillus subtilis caused by overproduction of the well-secreted AmyQ alpha-amylase from Bacillus amyloliquefaciens. Analyses of the control and overproducing strains were carried out at the end of exponential growth and in stationary phase, when protein secretion from B. subtilis is optimal. Among the genes...
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