Article
Multiplex PCR with minisequencing as an effective high-throughput SNP typing method for formalin-fixed tissue.
Electrophoresis - 1 Jul 2007
Gilbert Marcus T P, Sanchez Juan J, Haselkorn Tamara, Jewell Laurence D, Lucas Sebastian B, Van Marck Eric, Børsting Claus, Morling Niels, Worobey Michael
Abstract excerpt
Extensive collections of formalin-fixed paraffin-embedded (FFPE) tissues exist that could be exploited for genetic analyses in order to provide important insights into the genetic basis of disease or host/pathogen cointeractions. We report here an evaluation of a 44 SNP multiplex genotyping method, multiplex PCR with minisequencing (MPMS), on 92 DNA extractions performed on six archival FFPE samples of variable...
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