Adenosine 5'-O-(3-thio)triphosphate (ATPgammaS) promotes positive supercoiling of DNA by T. maritima reverse gyrase.

Abstract excerpt

Reverse gyrases are topoisomerases that catalyze ATP-dependent positive supercoiling of circular covalently closed DNA. They consist of an N-terminal helicase-like domain, fused to a C-terminal topoisomerase I-like domain. Most of our knowledge on reverse gyrase-mediated positive DNA supercoiling is based on studies of archaeal enzymes. To identify general and individual properties of reverse gyrases, we set out...

Topics

• Adenosine Triphosphate
• Bacterial Proteins
• DNA Topoisomerases, Type I
• DNA, Superhelical
• Models, Molecular
• Mutation
• Nucleic Acid Conformation
• Nucleotides
• Plasmids
• Protein Structure, Tertiary
• Recombinant Proteins