Article
Functional evaluation of four putative DNA-binding regions in Thermoanaerobacter tengcongensis reverse gyrase.
Extremophiles : life under extreme conditions - 1 Mar 2011
Li Jie, Liu Jingfang, Zhou Jian, Xiang Hua
Abstract excerpt
Reverse gyrase (RG) is an ATP-dependent type I DNA topoisomerase that introduces positive supercoils into DNA in thermophiles. Four regions of RG, i.e., the N-terminal zinc-finger motif, the β-hairpin in subdomain H1, the "latch", and the C-terminal zinc-finger motif, were predicted to be involved in DNA binding previously. In this paper, the functions of these regions in the enzymatic activity were evaluated by...
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