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Article

"Mark the gene": a method for nondestructive introduction of marker sequences inside the gene frame of transgenes.

Applied and environmental microbiology - 1 Aug 2007

Morono Yuki, Kitagawa Wataru, Kimura Nobutada, Noda Naohiro, Nakamura Kazunori, Kamagata Yoichi

Abstract excerpt

A specific marking and detection technique is a fundamental requirement for the safer use of genetically modified (GM) organisms. Here we propose a simple and effective method for directly marking functional transgenes in GM organisms. For that purpose, we introduced nucleotide substitutions (NS), based on the degeneracy of codons as markers (NS markers), into the bphC (2,3-dihydroxybiphenyl dioxygenase) and...

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Identifiers

PMID: 17526781
DOI: 10.1128/AEM.00068-07
PMCID: PMC1951010

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Source receipt: pubmed, retrieved 7 Sept 2026.

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