Article
"Mark the gene": a method for nondestructive introduction of marker sequences inside the gene frame of transgenes.
Applied and environmental microbiology - 1 Aug 2007
Morono Yuki, Kitagawa Wataru, Kimura Nobutada, Noda Naohiro, Nakamura Kazunori, Kamagata Yoichi
Abstract excerpt
A specific marking and detection technique is a fundamental requirement for the safer use of genetically modified (GM) organisms. Here we propose a simple and effective method for directly marking functional transgenes in GM organisms. For that purpose, we introduced nucleotide substitutions (NS)...
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