Article
Development of a general method for detection and quantification of the P35S promoter based on assessment of existing methods.
Scientific reports - 8 Dec 2014
Wu Yuhua, Wang Yulei, Li Jun, Li Wei, Zhang Li, Li Yunjing, Li Xiaofei, Li Jun, Zhu Li, Wu Gang
Abstract excerpt
The Cauliflower mosaic virus (CaMV) 35S promoter (P35S) is a commonly used target for detection of genetically modified organisms (GMOs). There are currently 24 reported detection methods, targeting different regions of the P35S promoter. Initial assessment revealed that due to the absence of primer binding sites in the P35S sequence, 19 of the 24 reported methods failed to detect P35S in MON88913 cotton, and the...
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