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Article

A universal vector concept for a direct genotyping of transgenic organisms and a systematic creation of homozygous lines.

eLife - 15 Mar 2018

Strobl Frederic, Anderl Anita, Stelzer Ernst Hk

Abstract excerpt

Diploid transgenic organisms are either hemi- or homozygous. Genetic assays are, therefore, required to identify the genotype. Our AGameOfClones vector concept uses two clearly distinguishable transformation markers embedded in interweaved, but incompatible Lox site pairs. Cre-mediated recombination leads to hemizygous individuals that carry only one marker. In the following generation, heterozygous descendants...

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Identifiers

PMID: 29543587
DOI: 10.7554/eLife.31677
PMCID: PMC5854464

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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