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Article

A new isolation method of human limbal progenitor cells by maintaining close association with their niche cells.

Tissue engineering. Part C, Methods - 1 May 2011

Chen Szu-Yu, Hayashida Yasutaka, Chen Mei-Yun, Xie Hua Tao, Tseng Scheffer C G

Abstract excerpt

In human corneal epithelium, self-renewal and fate decision of stem cells are highly regulated in a niche microenvironment called palisades of Vogt in the limbus. Herein, we discovered that digestion with dispase, which cleaves off the basement membrane, did not remove the entire basal epithelial progenitor cells. In contrast, digestion with collagenase isolated on cluster consisting of not only entire epithelial...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Identifiers

PMID: 21175372
DOI: 10.1089/ten.TEC.2010.0609
PMCID: PMC3129703

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