Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

Isolation and expansion of human limbal stromal niche cells.

Investigative ophthalmology & visual science - 25 Jan 2012

Xie Hua-Tao, Chen Szu-Yu, Li Gui-Gang, Tseng Scheffer C G

Abstract excerpt

PURPOSE: Limbal stromal niche cells heterogeneously express embryonic stem cell (SC) markers. This study was conducted to isolate and expand them and to prove that their phenotype is critical for supporting SCs. METHODS: Human limbus was isolated by dispase or collagenase. Single cells were seeded on coated, 2D, or 3D Matrigel and were serially passaged in modified embryonic SC medium (MESCM), supplemented...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to Adult Stem Cells
Share to Drug Combinations
Share to Epithelium, Corneal

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Share to Fluorescent Antibody Technique, Indirect

Share to Laminin

Share to Limbus Corneae

Share to Proteoglycans

Share to Stem Cell Niche

Source

Open in PubMed Open full text

Identifiers

PMID: 22167096
DOI: 10.1167/iovs.11-8441
PMCID: PMC3292364

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 6 Sept 2026. Manifest publication-graph-cohort-2026-09-06.1.

Source receipt: pubmed, retrieved 6 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Isolation and expansion of human limbal stromal niche cells.PMID 22167096Read ↗

A new isolation method of human limbal progenitor cells by maintaining close association with their niche cells.Semantic proximity Read ↗

Mesenchymal cells from limbal stroma of human eye.Semantic proximity Read ↗

Human amniotic epithelial cells as novel feeder layers for promoting ex vivo expansion of limbal epithelial progenitor cells.Semantic proximity Read ↗

Preservation of the limbal stem cell phenotype by appropriate culture techniques.Semantic proximity Read ↗

Human Limbal Mesenchymal Cells Support the Growth of Human Corneal Epithelial Stem/Progenitor CellsSemantic proximity Read ↗

Stem Cell Activity in the Developing Human CorneaSemantic proximity Read ↗

Partial enrichment of a population of human limbal epithelial cells with putative stem cell properties based on collagen type IV adhesiveness.Semantic proximity Read ↗

Limbal stem cells: identity, developmental origin, and therapeutic potentialSemantic proximity Read ↗

Spatial Distribution of Niche and Stem Cells in Ex Vivo Human Limbal CulturesSemantic proximity Read ↗

Differentiation of Human Embryonic Stem Cells into Corneal Epithelial-Like Cells by In Vitro Replication of the Corneal Epithelial Stem Cell NicheSemantic proximity Read ↗

New characterization and safety evaluation of human limbal stem cells used in clinical application: fidelity of mitotic process and mitotic spindle morphologiesSemantic proximity Read ↗

Isolation and Characterization of Multipotent Cells from Human Fetal DermisSemantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.