Article
Yeast mutant with efficient secretion identified by a novel secretory reporter, Cluc.
Biochemical and biophysical research communications - 29 Jun 2007
Kanjou Naoko, Nagao Aki, Ohmiya Yoshihiro, Ohgiya Satoru
Abstract excerpt
Yeast is an important host for the production of pharmaceutical or industrial proteins by virtue of its genetic information and easy handling. A number of heterologous proteins have been produced and purified from yeast cell cultures as secreted forms. Here, we describe a novel screening system of Saccharomyces cerevisiae and its application to improve the secretion efficiency of yeast. In our system, a natural...
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