Article
Mutational analysis of the von Willebrand factor gene in type 1 von Willebrand disease using conformation sensitive gel electrophoresis: a comparison of fluorescent and manual techniques.
Haematologica - 1 Apr 2007
Hashemi Soteh Mohammad, Peake Ian R, Marsden Luke, Anson John, Batlle Javier, Meyer Dominique, Fressinaud Edith, Mazurier Claudine, Goudemand Jenny, Eikenboom Jeroen, Goodeve Anne
Abstract excerpt
Two versions of conformation sensitive gel electrophoresis, fluorescent (F-CSGE) and manual (M-CSGE) techniques, were compared for mutation analysis of the von Willebrand factor gene. 56 PCRs were used to amplify all 52 exons of the gene in seven type 1 von Willebrand disease cases, plus a healthy control. One hundred and ninety-two samples were analyzed on each F-CSGE gel, compared with 40 on M-CSGE. 125...
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